Online – VCE – Biology UNIT 4 : Tools and techniques for manipulating DNA

Introduction

How do humans impact on biological processes?

Students examine the impact of human culture and technological applications on biological processes. They apply their knowledge of the structure and function of the DNA molecule to examine how molecular tools and techniques can be used to manipulate the molecule for a particular purpose. Students describe gene technologies used to address human issues and consider their social and ethical implications. Scientific knowledge can both challenge and be challenged by society. Students examine biological challenges that illustrate how the reception of scientific knowledge is influenced by social, economic and cultural factors.

Program Overview

These programs are independent programs. You can choose to complete one or both.

Program 1 – PCR and Gel electrophoresis

Students complete pre-work on the theory of PCR and how to read electrophoresis gels. They then observe either a live remote lab/recording of pipetting the following into the PCR tube: DNA, primers, and DNA master mix. Students then observe the PCR program being started and the stages of PCR on the thermal cycler display. Next, the PCR samples are loaded into a gel and run with a time-lapse recording/prepared gel to observe the results. Students then use the images of pre-run gels to identify the segments of DNA.

Program 2 – Restriction enzyme and plasmids (using pGlo)

Students complete pre-work on the theory of restriction enzymes and plasmids. They then observe a live remote lab/recording of pipetting plasmids, buffer and restriction enzymes into a micro tube/s, which are then placed into a water bath in preparation to run a gel. Products from the micro tubes are then pipetted onto the gel (this is recorded as a time-lapse). While the gel runs, students observe the set up for bacterial transformations. Bacteria, plasmids and other required substances are pipetted into tubes which are then placed into a water bath, followed by an ice bath. At room temperature, the bacteria in the tubes are then plated and taped up. Students then use the images of pre-run gels to identify the segments of plasmid and DNA. We are hoping to send pGlo plates to your schools so students can observe the results of the plate bacterial growth.

Special Notes:

  1. KIOSC will conference (PL) with the classroom teacher to show them the activity.
  2. KIOSC will negotiate a time to run the LIVE sessions in the class.
  3. TEACHER Notes (complete with guide and all answers) and all other materials are included.
  4. The classroom teacher will need to provide student names and emails to KIOSC. This is so students can be given access to KIOSC’s Learning Management System.
Duration:

The online programs can each be completed in 2-3 hours. It is up to the classroom teacher how they would like KIOSC to deliver the program. Being flexible is possible.

Curriculum Focus

VCE Biology Unit 4 – AOS 2: Outcome 2: How do humans impact on biological processes?

Max Students:

Unlimited